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分子克隆实验指南（第四版）
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作　者：MICHAEL R.GREEN，JOSEPH SAMBROOK 著
ISBN：9出版时间：页数：2028
包装：平装开本：16开字数：
《分子克隆实验指南（第四版）》简介：　　Molecular Cloning has served as thefoundation of technical expertise in labs worldwide for 30 years.No other manual has been so popular， or so influential. MolecularCloning， Fourth Edition， by the celebrated founding author JoeSambrook and new co-author， the distinguished HHMI investigatorMichael Green， preserves the highly praised detail and clarity ofprevious editions and includes specific chapters and protocolscommissioned for the book from expert practitioners at Yale， UMass， Rockefeller University， Texas Tech， Cold Spring HarborLaboratory， Washington University， and other leading institutions.The theoretical and historical underpinnings of techniques areprominent features of the presentation throughout， information thatdoes much to help trouble-shoot experimental problems.For the fourth edition of this classic work，the content has been entirely recast to include nucleic-acid basedmethods selected as the most widely used and valuable in molecularand cellular biology laboratories.Core chapters from the third edition havebeen revised to feature current strategies and approaches to thepreparation and cloning of nucleic acids， gene transfer， andexpression analysis. They are augmented by 12 new chapters whichshow how DNA， RNA， and proteins should be prepared， evaluated， andmanipulated， and how data generation and analysis can behandled.The new content includes methods for studyinginteractions between cellular components， such as microarrays，next-generation sequencing technologies， RNA interference， andepigenetic analysis using DNA methylation techniques and chromatinimmunoprecipitation. To make sense of the wealth of data producedby these techniques， a bioinformatics chapter describes the use ofanalytical tools for comparing sequences of genes and proteins andidentifying common expression patterns among sets of genes.Building on thirty years of trust， reliability，and authority， the fourth edition of Molecular Cloning is the newgold standard—the one indispensable molecular biology laboratorymanual and reference source. 新版特点：？ Extensive new content： 12 entirely new chapters are devoted tothe most exciting current research strategies， including epigeneticanalysis， RNA interference， genome sequencing， andbioinformatics？ Expanded scope： the nucleic-acid-based techniques selected forinclusion have promoted recent advances in gene transfer， proteinexpression， RNA analysis， and expression of cloned genes？ Classic content： 10 original core chapters have been updatedto reflect developments and innovations in standard techniques andto introduce new cutting-edge protocols？ Easy-to-follow format： the previous editions’ renowned attentionto detail and accuracy are fully retained？ Essential appendices： an up-to-date collection of reagents，vectors， media， detection systems， and commonly used techniques areincluded？ Expanded authorship： chapters and protocols have beenspecifically commissioned from renowned experts at leadinginstitutions
暂缺《分子克隆实验指南（第四版）》作者简介
《分子克隆实验指南（第四版）》目录：VOLUME&1Part&1：&Essentials1.&Isolation&and&Quantification&of&DNA2.&Analysis&of&DNA3.&Cloning&and&Transformation&with&Plasmid&Vectors4.&Gateway&Recombinational&CloningJohn&S.&Reece-Hoyes&and&Albertha&J.M.&Walhout5.&Working&with&Bacterial&Artificial&Chromosomes&and&OtherHigh-Capacity&VectorsNathaniel&Heintz&and&Shiaoching&Gong6.&Extraction，&Purification，&and&Analysis&of&RNA&from&EukaryoticCells7.&Polymerase&Chain&Reaction8.&BioinformaticsJui-Hung&Hung&and&Zhiping&WengVOLUME&2Part&2：&Analysis&and&Manipulation&of&DNA&and&RNA9.&Quantification&of&DNA&and&RNA&by&Real-Time&Polymerase&ChainReaction10.&Nucleic&Acid&Platform&TechnologiesOliver&Rando11.&DNA&SequencingElaine&Mardis&and&W.&Richard&McCombie12.&Analysis&of&DNA&Methylation&in&Mammalian&CellsPaul&M.&Lizardi，&Qin&Yan，&and&Narendra&Wajapeyee13.&Preparation&of&Labeled&DNA，&RNA，&and&OligonucleotideProbes14.&Methods&for&In&Vitro&MutagenesisMatteo&Forloni，&Alex&Liu，&and&Narendra&WajapeyeePart&3：&Introducing&Genes&into&Cells15.&Introducing&Genes&into&Cultured&Mammalian&CellsPriti&Kumar，&Arvindhan&Nagarajan，&and&Pradeep&D.&Uchil16.&Introducing&Genes&into&Mammalian&Cells：&Viral&VectorsGuangping&Gao&and&Miguel&Sena-EstevesVOLUME&3Part&4：&Gene&Expression17.&Analysis&of&Gene&Regulation&Using&Reporter&SystemsPradeep&D.&Uchil，&Arvindhan&Nagarajan，&and&Priti&Kumar18.&RNA&Interference&and&Small&RNA&AnalysisChengjian&Li&and&Phillip&D.&Zamore19.&Expressing&Cloned&Genes&for&Protein&Production，&Purification，and&AnalysisClara&L.&Kielkopf，&William&Bauer，&and&Ina&UrbatschPart&5：&Interaction&Analysis20.&Cross-Linking&Technologies&for&Analysis&of&Chromatin&Structureand&FunctionTae&Hoon&Kim&and&Job&Dekker21.&Mapping&of&In&Vivo&RNA-Binding&Sites&by&UV-Cross-LinkingImmunoprecipitation&（CLIP）Jennifer&C.&Darnell，&Aldo&Mele，&Ka&Ying&Sharon&Hung，&and&Robert&B.Darnell22.&Gateway-Compatible&Yeast&One-Hybrid&and&Two-Hybrid&AssaysJohn&S.&Reece-Hoyes&and&Albertha&J.M.&WalhoutAppendices1.&Reagents&and&Buffers2.&Commonly&Used&Techniques3.&Detection&Systems4.&General&Safety&and&Hazardous&Material
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中文名: 分子克隆实验指南(上册)
发行时间: 日
资源格式: PDF
资源版本: 第3版
译者: 黄培堂
地区: 大陆
语言: 简体中文
分子克隆实验指南(上册) 简介：
分子克隆实验指南(上册) 内容介绍：
《克隆实验指南》的前两版以其无克匹敌的声誉，在近20年的时间里一直被作为分子生物学实验的经典参考书。《分子克隆实验指南》的作者该对图书内容进行了完全的升级，修订了实验的每条方案，增加了大量新的材料，拓宽了它涉及的领域。
《分子克隆实验指南》具有先进性、实用性、权威性的特点，是生命科学实验室内当之无愧的&圣经&。《分子克隆实验指南》的内容丰富而详细，使其具有用于学习遗传学、分子细胞生物学、发育生物学、微生物学、神经科学和免疫学等科学的重要指导和参考价值。
分子克隆实验指南(上册) 目录：
第1章 质粒及其在分子克隆中的应用
第2章 &噬菌体及其载体
第3章 M13噬菌体载体
第4章 高容量载体的应用
第5章 DNA凝胶电泳和脉冲场琼脂糖凝胶电泳
第6章 真核基因组DNA的制备和分析
第7章 真核mRNA的提取、纯化和分析
第8章 聚合酶链式反应体外扩增DNA
第9章 放射性标记DNA探针与RNA探针的制备
第10章 合成寡核苷酸探针
第11章 cDNA文库制备及其基因鉴定
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