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小麦族仲彬草属植物的细胞学和生化标记以及ITS序列分析
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小麦族仲彬草属植物的细胞学和生化标记以及ITS序列分析.PDF
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出门在外也不愁&&&信号肽序列
的翻译结果:
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&在分类学科中查询
The five genes were predicted products of 338, 335, 336, 343 and 346 amino acid residues, respectively, including putative signal peptide sequence at the amino
termini. And OSP1, OSP4 and OSP5 were predicted to be anionic peroxidase, OSP2 and OSP3 are cationic.
该 5个基因分别编码 338、335、336、343和 346个氨基酸残基的蛋白质 ,而且都具有N端信号肽序列 ,其中OSP1、OSP4、OSP5为阴离子过氧化物酶 ,OSP2、OSP3为阳离子过氧化物酶。
Ubiquitin and signal peptide sequence of pathogenesis-related protein 1a(PRS) were recombined together by TP-PCR in the research based on pBin438 of the efficient strongly plant expression vector.
本研究是在植物高效表达载体pBin438的基础上,采用三引物PCR法(TP-PCR)技术,将拟南芥(Arabidopsis)泛素基因与烟草病程相关蛋白PR1a基因的信号肽序列体外重组;
Methods The recombinant retrovirus vector pSIV-1/hIL-17F(including the 20aa signal peptide sequence,moreover,hIL-17F gene inside GAATTC→GAACTC synonymous mutation)was constructed by PCR according to pUCm-T/hIL-17F and identified by PCR,double endonuclease digestion and DNA sequencing.
方法以测序验证的pUCm-T/hIL-17F为模板,采用聚合酶链式反应(PCR)技术扩增目的基因片段(包括20aa信号肽序列,而且hIL-17F基因内EcoR I酶切位点GAA T TC→GAA C TC同义点突变,即AA T→AA C,均为Asn),并正向插入逆转录病毒载体pSIV-1后经PCR、双酶切和测序鉴定。
The cDNA of ea4 gene was 519 bp in length,in which 1-48 bp was the signal peptide sequence. And the amino acid sequence encoded by 49-519 bp showed 98% similarity with that of EA4 protein,with 3 amino acids difference at the C-terminal,in which the 156th was the first defined one.
ea4基因的cDNA全长519 bp,其中1-48 bp为信号肽序列,49-519 bp编码的氨基酸与蛋白质纯化后测序EA4部分的一致性为98%,C末端存在3个氨基酸的差异,其中第156位为新确定氨基酸。
The Application of Signal Peptide Sequence in Yeast Secretory Expression System
信号肽序列在酵母分泌表达系统中的应用
Methods BCG Ag85B signal sequence and IFN-α2a gene were amplified from the genome of BCG and IFNα2a by PCR,respectively.
方法分别以卡介苗(BCG)和IFN-α2a cD-NA为模板,通过PCR扩增得到约117bp的BCG-Ag85B信号肽序列和495bp的IFN-α2a基因序列。
To construct a recombinant secretory BCG Ag85B fused human interleukin (IL)
BCG Ag85B signal sequence and IL
2 gene were amplified from the genome of BCG and IL
2 by PCR, respectively.
目的　构建分泌性表达白介素 (IL)
2的重组卡介苗 (BCG)。 方法　分别以BCG和IL 2cDNA为模板 ,通过PCR扩增得到约 117bp的BCG抗原 85B(BCG Ag85B)信号肽序列和 399bp的IL 2基因序列。
BCG Ag85B signal sequence was cloned in E. coli
BCG shuttle
vector pMV261 to obtain pMS.
将BCG Ag85B信号肽序列克隆至大肠杆菌
BCG穿梭质粒 pMV2 6 1,得到重组质粒 pMS。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增IL_3信号肽序列和人内皮抑素基因,然后采用重叠延伸PCR拼接的方法将两者拼接和扩增。
Taking advantage of the restriction endonuclease NcoⅠof S1 gene and pET-22b,the signal sequence and some hydrophobic part of S1 gene were cut off to get another recombinant vector pET-22b-SNS.
利用载体和S1基因上的NcoⅠ酶切位点,将S1基因的信号肽序列和部分疏水序列切掉后,构建pET-22b-SNS重组质粒。
The V_H and V_L genes of mAb 5C11 and relative signal peptide sequences were spliced with C_H and C_κ genes of human IgG1 to construct expression plasmid pIRES/h5C11 of human-mouse chimeric antibody gene and the plasmid was transfected into 293T cells under Lipofectamine mediation for transient expression.
利用基因重组技术,将mAb5C11的VH、VL基因及其相应信号肽序列与人IgG1的CH基因、Cκ链基因进行拼接,构建人-鼠嵌合抗体基因的表达质粒pIRES/h5C11。 用脂质体法将其导入293T细胞株中进行瞬时表达。
Influence of Signal Peptide Sequences on the Expression of Heterogeneous Proteins in Pichia pastoris
信号肽序列对毕赤酵母表达外源蛋白质的影响
products by half
nested PCR using signal peptide sequences as primers were superior in quality
and quantity to those by PCR with conserved sequences in the 5′
end variable regions as primers.
以免疫球蛋白信号肽序列为引物进行半套式PCR所得到的产物在质和量上都优越于以可变区5′末端保守序列为引物进行PCR所得到的产物。
To ensure secretion of PEX by the target cells, the signal peptide sequences of 87bp MMP2 were included in the upstream of PEX cDNA, then the expression vector pcDNA3.1-sig-PEX was constructed, the anti-tumor effect in vitro and in vivo was observed and its biological effects were studied .
MMP-2的氨基端本身存在87bp的信号肽序列（Sig），而且此分泌信号肽有着较高水平表达，因此本实验采用与蛋白同源的分泌信号。
According to sequence analysis, the primer was designed to amplify signal peptide sequences relative to V_H and V_L genes.
根据序列分析的结果,设计引物扩增VH和VL基因相应的信号肽序列。
Internet tools and signal sequence trap could discern the signal sequences.
可以利用因特网在线工具和信号序列捕获系统来判定基因序列中是否含有信号肽序列。
In this study the pUC 19 plasmid was used as a backbone and the regulatory and signal sequences of BCG extracellular antigen 85-B,and Neo gene sequence,which encodes kanamycin resistance, and a mycobacterial plasmid origin of replication(Ori M)were progressively cloned into the pUC19 plasmid. The recombinant E.
本研究以pUC19为骨架，分别克隆入BCG分泌抗原85－B的调节和信号肽序列、Neo基因序列（编码卡那霉素抗性）以及分枝杆菌质粒复制基因，构建成分枝杆菌－大肠杆菌穿梭质粒pBCG－8000。
At first, the Saccharomyces cerevisiae mating factor α prepro-leader sequence was synthesized using successive PCR and designated as MF4I. Then, ten different signal sequences were constructed by adding the N-terminal residues of Pichia pastoris Aox1 protein to the N-terminal of the MF4I. These ten signal sequences were used for directing phytase gene secretion in Pichia pastoris, the secretion of phytase were increased in Pichia pastoris strains containing new signal sequence.
首先按毕赤酵母的偏爱密码合成了酿酒酵母的α因子信号肽序列MF4I,随后在MF4I信号肽序列的N端分别引入 1～ 10个毕赤酵母Aox1蛋白质的N端氨基酸 ,构成 10种不同的分泌信号肽序列 ,10种不同的分泌信号肽序列被用于植酸酶基因的毕赤酵母分泌表达。
METHODS: Human immunoglobulin heavy chain and light chain genes were amplified respectively by RT-PCR from different human lymphocytes using family specific primers and signal sequences of immunoglobulin as half-nested PCR primers.
方法:从不同人群外周血淋巴细胞中提取总RNA,经反转录后,以免疫球蛋白信号肽序列引物和家族特异性免疫球蛋白可变区基因引物,进行半套式PCR扩增人全套抗体基因片段,并克隆于pComb3H载体,电转化大肠杆菌XL1-Blu,在辅助噬菌体的超感染下,构建噬菌体抗体组合文库。
For further improving the secretion expression of heterogeneous proteins, in this research, the signal sequences were studied.
为了进一步提高外源蛋白质的分泌表达 ,对信号肽序列进行了研究。
查询“信号肽序列”译词为用户自定义的双语例句&&&&我想查看译文中含有：的双语例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& A cDNA library was prepared from pacific Chinook Salmon pituitaries.Salmon prolactin gene was screened using synthetic oligonucleotide probes based on partial protein sequence A positive clone (PRL-10) was identified and sequenced.It is a full size clone containing 1.1 kb and coding for a preprolactin of 211 amino acids. &&&&&&&&&&&&从太平洋切奴克鲑鱼的垂体制备cDNA文库。按照鲑鱼泌乳素的部分蛋白质序列所提供的信息合成寡聚脱氧核苷酸探针。用探针筛查泌乳素基因,识别出一个阳性克隆PRL-10。该克隆的硷基序列已被测出。PRL-10的总长为1.1kb,编码了含有211个氨基酸组成的泌乳素前体,其中包括了编码23个氨基酸的信号肽序列和编码188个氨基酸的成熟泌乳素序列。&&&&&&&& By the method of oligonucleotide-directed mutagenesis, we made 27bp and 18bp deletion at junction region between a-factor signal sequence and α-hANP gene, α-factor signal sequence and α-IFN gene, respectively. Since the deleted region contains one WindⅢ site, the mu-iams without this site were selected. The result of DNA sequence analysis showed that the sequences of the mutants were the same as designed. &&&&&&&&&&&&本实验采用寡聚核苷酸指导的定点突变法,缺失了分别存在于YFD42和YFD58中的a-因子信号肽序列与a-hANP基因和a-因子信号肽序列与a-1FN基因间接头区域的27和18个核苷酸。由于被缺失部分恰好含有一个酶切位点,利用这一特点,酶切检查初步筛选出缺失了一个HindⅢ酶切位点的突变子。经DNA序列分析,证实缺失的核苷酸序列和设计完全一致。&&&&&&&& Using oligonucleotide directed mutagenesis techniques, the cDNA gene of human bone morphogenetic protein (hBMP~l) was designed for reforming to express in E. coli. The leading sequence located at 5'-end of the gene was deleted and translational starting codon with a restriction endonuclease Ncol site was introduced .The reformed gene was inserted into plasmid pBR322 and recombinant was transformed with E. coli HB101- Analysis with restriction enzyme mapping and DNA sequencing shows that the gene mutagenesis... &&&&&&&&&&&&用寡核苷酸诱导的定位突变技术,将骨形成蛋白基因5’端经突变后去除了信号肽序列并,引入限制性内切酶NcoI位点及起始密码ATG,将此片段重新组装后克隆入pBR322质粒中,为进一步使基因克隆和表达有活性的骨形成蛋白奠定了基础。&nbsp&&&&&&&&相关查询:
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